| Title |
Fig. 1. Knockout of six Hsp70 family genes reduces the median lifespan of D. melanogaster and has a pronounced negative effect on the speed of locomotion in the negative geotaxis test. a – Normalized number of reads (RNA sequencing) per open reading frame of each gene. n = 12 pools (legs of 15 flies) of each line. b and c – Proportion of surviving flies with normal and chronically elevated levels of motor (locomotor) activity. The p value is shown for comparison of curves, as well as for the median and maximum lifespan (proportion of survivors 50 and 10%); n > 150 in each line. TP – training. d – Age-related changes in the speed of locomotion in the negative geotaxis test. One and three asterisks represent p values < 0.05 and < 0.001, respectively. n = 5–6 pools (10–30 flies per pool) of each line. |