The authors use palynological method in order to refine chronostratigraphy on multilayered archeological site Os’kino Boloto (forest-steppe Tobol basin). Pollen data of the settlement profile were compared with the samples taken under Tashkovo’s vessel (early Bronze Age) and under Koptyaki’s vessel (middle Bronze Age). The results show that different cultures can be stratified with the help of the palynological analysis - however, the research also highlighted some complications of correlating the data, obtained from the profile and the probe results. In the early Bronze Age, the birch and pine forests were very common with a, diverse range of herbaceous species. In middle Bronze Age, the birch became dominated and herbaceous got less various. In general, the results, obtained from pollen analysis matched previous studies, in the Tobol basin territory. Considering analysis of samples under the vesses, pollen spectra from Tashkovo’s vessel didn’t match the profile pollen spectra. Dominance of pine pollen might be related to pine branches, stored in a dwelling. The data obtained from under Koptyaki’s vessel match the settlement profile results.