Influence of model pollutants contained in drinking water on the metabolic activity of the human duodenal adenocarcinoma cell line HuTu-80

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Abstract

Introduction. Currently, a promising direction in water sample toxicity research involves conducting screening analysis on human cell lines.
The purpose of this study is to assess the impact of model drinking water pollutant on the metabolic activity of the human duodenal adenocarcinoma cell line HuTu-80 to justify its further use as a test object for evaluation the toxicity of drinking water samples.
Materials and methods. The research object is the standard culture of the duodenal adenocarcinoma cell line HuTu-80. To assess the toxic effect of chloride, sulfate, and nitrate ions on the cell culture, their sodium salts were used: NaCl in concentrations ranging from 100 to 10000 mg/L, Na2SO4 from 20 to 2000 mg/L, and NaNO3 from 2 to 200 mg/L. The toxic effects of pollutants on the HuTu-80 cell culture were determined using the MTT assay. Statistical analysis of the data was conducted using the Statistica 10 software (Statsoft, USA).
Results. The study evaluating the influence of model drinking water pollutants on the metabolic activity of the HuTu-80 duodenal adenocarcinoma cell culture revealed a dose-dependent effect of chloride, sulfate, and nitrate ions, resulting in a decrease in their metabolic activity.
Limitations. The application of this research method is feasible only in a specially equipped laboratory with qualified personnel.
Conclusion. The data obtained from this study may serve as a basis for the development of a test system utilizing the human intestinal cell culture to assess the toxicity of drinking water samples.

About the authors

Irina A. Mamonova

Saratov State Medical University named after V.I. Razumovsky

Email: mamonova.83@rambler.ru
PhD (Biology), senior lecturer of the Department of microbiology, virology, and immunology, Saratov State Medical University named after V.I. Razumovsky, Saratov, 410012, Russian Federation

Dmitry A. Kuzyanov

Saratov Hygiene Medical Research Center of the Federal Scientific Center for Medical and Preventive Health Risk Management Technologies

Email: dimakuzyanov2000@gmail.com
junior researcher at the Laboratory of Chemical and Biological Monitoring of Water Quality, Saratov Hygiene Medical Research Center of the Federal Scientific Center for Medical and Preventive Health Risk Management Technologies, Saratov, 410022, Russian Federation

Irina S. Kosheleva

Saratov Hygiene Medical Research Center of the Federal Scientific Center for Medical and Preventive Health Risk Management Technologies

Email: irishka-kosheleva@mail.ru
junior researcher at the Laboratory of Chemical and Biological Monitoring of Water Quality, Saratov Hygiene Medical Research Center of the Federal Scientific Center for Medical and Preventive Health Risk Management Technologies, Saratov, 410022, Russian Federation

Leonid P. Erdniev

Saratov Hygiene Medical Research Center of the Federal Scientific Center for Medical and Preventive Health Risk Management Technologies

Email: leonid-erdniev@yandex.ru
PhD (Medicine), researcher at the Laboratory of Chemical and Biological Monitoring of Water Quality, Saratov Hygiene Medical Research Center of the Federal Scientific Center for Medical and Preventive Health Risk Management Technologies, Saratov, 410022, Russian Federation

Arina S. Evsyukova

N.G. Chernyshevsky Saratov National Research State University; Institute of Biochemistry and Physiology of Plants and Microorganisms

Email: arina-evsyukova@mail.ru
engineer, N.G. Chernyshevsky Saratov National Research State University, Saratov, 410012, Russian Federation

Alexander A. Shirokov

Saratov State Medical University named after V.I. Razumovsky; N.G. Chernyshevsky Saratov National Research State University; Institute of Biochemistry and Physiology of Plants and Microorganisms

Email: shirokov_a@ibppm.ru
PhD (Biology), head of the Center for Collective Use “Symbiosis”, Saratov State Medical University named after V.I. Razumovsky, Saratov, 410012, Russian Federation

Larisa Yu. Matora

Institute of Biochemistry and Physiology of Plants and Microorganisms

Email: matora_l@ibppm.ru
DSc (Biology), head of the Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre, Saratov, 410012, Russian Federation

Yuri S. Gusev

Saratov Hygiene Medical Research Center of the Federal Scientific Center for Medical and Preventive Health Risk Management Technologies; N.G. Chernyshevsky Saratov National Research State University; Institute of Biochemistry and Physiology of Plants and Microorganisms

Email: yuran1989@yandex.ru
Ph.D., head of the Laboratory of Chemical and Biological Monitoring of Water Quality, Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre, Saratov, 410012, Russian Federation

Anatoly N. Mikerov

Saratov State Medical University named after V.I. Razumovsky; Saratov Hygiene Medical Research Center of the Federal Scientific Center for Medical and Preventive Health Risk Management Technologies

Email: mikerov@smncg.ru
DSc (Biology), head of the Saratov Hygiene Medical Research Center of the Saratov State Medical University named after V.I. Razumovsky, Saratov, 410012, Russian Federation

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