Rhodococcus qingshengii GlMm1 as the Basis of a Biosensor for Determination of the Fungicide Carbendazim

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Abstract

The possible application of Rhоdococcus qingshengii strain GlMm1, isolated from a Dead Sea clay sample, as the basis of a biosensor for determining the benzimidazole fungicide carbendazim was investigated. High sensitivity of the biosensor under neutral pH and up to 500 mM NaCl at 2 to 160 μM carbendazim was maintained for up to 30 days.

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About the authors

T. N. Kuvichkina

G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”

Email: lenokap80@gmail.com
Russian Federation, Pushchino, 142290

E. N. Kaparullina

G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”

Author for correspondence.
Email: lenokap80@gmail.com
Russian Federation, Pushchino, 142290

N. V. Doronina

G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”

Email: lenokap80@gmail.com
Russian Federation, Pushchino, 142290

A. N. Reshetilov

G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”

Email: lenokap80@gmail.com
Russian Federation, Pushchino, 142290

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2. Fig. 1. Calibration dependence of the biosensor based on the BcI of Rhodococcus qingshengii GlMm1 on the concentration of carbendazim. Biosensor response in picoamps per second (pA/s).

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3. Fig. 2. Substrate specificity of the BcI strain Rh. qingshengii GlMm1. Designations: 1 — catechol; 2 — fructose; 3 — L(‒) ‒ arabite; 4 — D-glucose; 5 — D-sorbitol; 6 ‒ D(+)-cellobiose; 7 — methylamine; 8 — dimethylamine; 9 ‒ carbendazim (substrate concentration 0.1 mM). Biosensor response in picoamps per second (pA/s).

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