New Bacteriophage Pseudomonas Phage Ka1 from a Trivia of Lake Baikal

M. S. Fedorova; Федорова М. С.; A. E. Gatina; Гатина А. Э.; V. N. Ilyina; Ильина В. Н.; L. L. Yadykova; Ядыкова Л. Л.; V. V. Dryukker; Дрюккер В. В.; A. S. Gorshkova; Горшкова А. С.; A. R. Kayumov; Каюмов А. Р.; E. Y. Trizna; Тризна Е. Ю.

doi:10.31857/S0026365624040126

New Bacteriophage Pseudomonas Phage Ka1 from a Trivia of Lake Baikal

Authors: Fedorova M.S.¹, Gatina A.E.¹, Ilyina V.N.¹, Yadykova L.L.¹, Dryukker V.V.², Gorshkova A.S.², Kayumov A.R.¹, Trizna E.Y.¹
Affiliations:
1. Kazan (Volga Region) Federal University
2. Limnological Institute, Siberian Branch of the Russian Academy of Sciences
Issue: Vol 93, No 4 (2024)
Pages: 474-480
Section: SHORT COMMUNICATIONS
URL: https://edgccjournal.org/0026-3656/article/view/655098
DOI: https://doi.org/10.31857/S0026365624040126
ID: 655098

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Abstract

The bacteriophage Pseudomonas phage Ka1 was isolated from the water of a tributary of Lake Baikal, in the area of discharge of treated wastewater in the city of Slyudyanka. The bacteriophage genome is 46,092 bp in size. and 50% composition of G + C pairs (AN OP455935.1) has 91% identity with the genome of Pseudomonas phage PSA37, belonging to the Bruynoghevirus from the class Caudoviricetes, which characterizes it as a new representative of Luz24-like phages (Bruynoghevirus). The bacteriophage lyses 62% of clinical isolates of Pseudomonas aeruginosa and is able to increase the effectiveness of gentamicin, ciprofloxacin, imipinem and meropenem by 4–8 times against this bacterium. Integrases, transposases and recombinases are not identified in the genome, which makes Pseudomonas phage Ka1 possible for use in complex therapy of infections caused by P. aeruginosa.

Keywords

bacteriophage, phage therapy, antibiotic resistance, Pseudomonas aeruginosa

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