Umbilical blood as a trophic-growth supplement for cultural work

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Abstract

This review analyzes the results of modern high-tech research on the use of umbilical cord blood serum/plasma as an additive to culture media for cell culture growth. Since culture media are a key factor in cell culture, the review addresses the composition and properties of the major culture media used in cell biology and regenerative medicine. The authors pay special attention to growth factors; they describe the functional properties of the main families of these polypeptides (fibroblast growth factors, epidermal growth factors, transforming growth factors, differentiation growth factors, epidermal growth factors, endothelial cell growth factors, hematopoietic growth factors, etc.). It was found that one of the most promising sources of growth factors is cord blood serum/plasma. In this publication, the main technologies for cord blood collection and systematic studies on the content of growth factors, cytokines, exosomes and mRNA in cord blood are presented. Experimental data on the use of umbilical cord blood serum/plasma as an additive to culture media for the growth of various cell cultures of animal origin are described. Human umbilical cord blood serum/plasma is an affordable, safe product with a high content of biologically active molecules compared to animal sources. In order for umbilical cord blood serum/plasma to be widely used as an adjunct to culture media, standards for the manufacture and testing of this product must be developed.

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About the authors

A. G. Goncharov

Immanuel Kant Baltic Federal University

Author for correspondence.
Email: larisalitvinova@yandex.ru

Center of Immunology and Cell Biotechnology

Russian Federation, Kaliningrad, 236041

V. V. Shupletsova

Immanuel Kant Baltic Federal University

Email: larisalitvinova@yandex.ru

Center of Immunology and Cell Biotechnology

Russian Federation, Kaliningrad, 236041

N. D. Gazatova

Immanuel Kant Baltic Federal University

Email: larisalitvinova@yandex.ru

Center of Immunology and Cell Biotechnology

Russian Federation, Kaliningrad, 236041

O. B. Melashchenko

Immanuel Kant Baltic Federal University

Email: larisalitvinova@yandex.ru

Center of Immunology and Cell Biotechnology

Russian Federation, Kaliningrad, 236041

K. A. Yurova

Immanuel Kant Baltic Federal University

Email: larisalitvinova@yandex.ru

Center of Immunology and Cell Biotechnology

Russian Federation, Kaliningrad, 236041

L. S. Litvinova

Immanuel Kant Baltic Federal University; Siberian State Medical University

Email: larisalitvinova@yandex.ru

Center of Immunology and Cell Biotechnology; Laboratory of Cellular and Microfluidic Technologies

Russian Federation, Kaliningrad, 236041; Tomsk, 634050

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