Structural and functional features of protein-polysaccharide complexes based on cysteine proteases and hydrophilicly modified chitosan
- Autores: Lavlinskaya M.S.1, Sorokin A.V.1, Goncharova S.S.1, Holyavka M.G.1,2, Kondratyev M.S.1,3, Artyukhov V.G.1
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Afiliações:
- Voronezh State University
- Sevastopol State University
- Institute of Cell Biophysics of the RAS
- Edição: Volume 50, Nº 5 (2024)
- Páginas: 612-621
- Seção: Articles
- URL: https://edgccjournal.org/0132-3423/article/view/670793
- DOI: https://doi.org/10.31857/S0132342324050047
- EDN: https://elibrary.ru/LRRAID
- ID: 670793
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Resumo
In this work, complexes of cysteine proteases, namely bromelain, papain and ficin, with a graft copolymer of chitosan and poly(N,N-dimethyl amino ethyl methacrylate) were obtained. It was determined that the enzyme catalytic activity in the complexes is reduced compared to their native forms. The results of molecular docking showed that modified polysaccharide located in the catalytic pocket of cysteine proteases globules. The resulting complexes have increased stability when stored under physiological conditions, which makes them promising candidates for use in the development of treatments for wounds.
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Sobre autores
M. Lavlinskaya
Voronezh State University
Email: holyavka@rambler.ru
Rússia, Universitetskaya Ploshchad 1, Voronezh, 394018
A. Sorokin
Voronezh State University
Email: holyavka@rambler.ru
Rússia, Universitetskaya Ploshchad 1, Voronezh, 394018
S. Goncharova
Voronezh State University
Email: holyavka@rambler.ru
Rússia, Universitetskaya Ploshchad 1, Voronezh, 394018
M. Holyavka
Voronezh State University; Sevastopol State University
Autor responsável pela correspondência
Email: holyavka@rambler.ru
Rússia, Universitetskaya Ploshchad 1, Voronezh, 394018; Universitetskaya ul. 33, Sevastopol, 299053
M. Kondratyev
Voronezh State University; Institute of Cell Biophysics of the RAS
Email: holyavka@rambler.ru
Rússia, Universitetskaya Ploshchad 1, Voronezh, 394018; Institutskaya ul. 3, Pushchino, 142290
V. Artyukhov
Voronezh State University
Email: holyavka@rambler.ru
Rússia, Universitetskaya Ploshchad 1, Voronezh, 394018
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