MORPHOLOGICAL EVALUATION IN EXPERIMENT OF BRAIN DAMAGES IN ACUTE POISONING WITH ETHANOL AND THEIR CORRECTION WITH PEPTIDE DRUGS

Results of morphological studies of brain after acute poisoning with ethanol and its correction with combination of molixan and semax are reported. 40% ethanol solution was intragastrically administered at a dose of 12 g / kg, which corresponded to 1.5 LD50. Semax was intranasally administered in a dose of 3 mg / kg. Molixan was administrated intraperitoneally in a dose of 30 mg / kg. The efficacy of peptide drugs was evaluated according to four application schemes: prophylactic (once 1 hour before ethanol), prophylactic and treatment (1 h prior to and immediately after administration of ethanol), early treatment (immediately after administration of ethanol and then once daily for 2 consecutive days), and delayed treatment (30 min after administration of ethanol and then once daily for 2 consecutive days). The preparation of material for the morphological study was performed 1 hour, 3 hours, 1 day and 3 days after modeling of alcohol intoxication. It was found out that ethanol at a dose of 1.5 LD50 causes congestion and stasis in the blood vessels of the pia mater, plasma-mpregnation of endothelium, microvascular damage to the blood-brain barrier that is followed by increase in their permeability, penetration of erythrocytes outside blood vessel walls, perivascular edema in brain tissue, change in shape and size of neurons (wrinkling of neurons). It was shown that the prophylactic and treatment application of the molixan and semax combination causes a reduction of ethanol toxic effects, which is manifested in significantly decreased plasmorrhagia severity and in the absence of wrinkled neurons.

poisoning, ethanol, molixan, semax, brain, morphological structure