Application of Flow Cytometry for Viability Assessment of Mutants for Translation Termination Factors in the Yeast Saccharomyces cerevisiae

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Abstract

Nonsense mutations in the essential SUP45 and SUP35 genes, encoding translation termination factors, affect the viability of Saccharomyces cerevisiae cells. Flow cytometry revealed that the viability of mutants was 3.5‒4 times lower compared to the wild-type. Moreover, the mutants were found to have higher sensitivity to ultrasonic treatment.

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About the authors

E. P. Efremova

St. Petersburg State University

Email: g.zhuravleva@spbu.ru
Russian Federation, St Petersburg, 199034

O. M. Zemlyanko

St. Petersburg State University; Laboratory of Amyloid Biology SPBU

Email: g.zhuravleva@spbu.ru
Russian Federation, St Petersburg, 199034; St Petersburg, 199034

G. A. Zhouravleva

St. Petersburg State University; Laboratory of Amyloid Biology SPBU

Author for correspondence.
Email: g.zhuravleva@spbu.ru
Russian Federation, St Petersburg, 199034; St Petersburg, 199034

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2. Fig. 1. Mutations in the SUP45 and SUP35 genes lead to a decrease in viability. The data of flow cytofluorometry (single cell populations) for clones carrying alleles SUP45 (panel A) or SUP35 (panel B) are presented. FSC-A is a parameter of direct light scattering by area. FL3-A::PI-A is a fluorescent detection channel (610/20 nm) that registers the intensity of the fluorescent signal (in this case, propidium iodide) by area.

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